DNA

Part:BBa_K2557042:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


Bxb1 attP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 62


Design Notes

To facilitate cloning, we randomly added some bases on both sides of attP.


Source

After the recombination site of Bxb1 was found in the literature, it was sent to Kingsray for synthesis.

References